Meanwhile 16 professionals will also take part in the next round following their qualifier on Friday.
Abraham Ainamani finished second, four strokes away while Saidi Mawa and Robert Oluba tied for third place.
Madoya shot a third round level-par 71, to maintain his lead, going into the final round of the Safari Tour event at the par-71 course.
Opio who slept in second position after the opening round behind Herman ‘Deco’ Mutebi improved her first round score by a stroke to shoot to the top.
The 20-year old returned 153 gross over 36 holes to beat over 100 caddies from BOTH, Uganda and Kenya
Golfers will try their luck on holes 6 and 12.The tournament provides a test of skill and ability
The tournament is aimed at supporting struggling clubs in the country
The winning pair from every month will advance to the challenge finale in June.
Senior golfers above 55 years are among a record 190 golfers that registered.
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