The 29-year-old, who joined from Bayern Munich this month, missed Monday's 3-1 win over Arsenal and will now self-isolate.
Pep Guardiola's side went ahead after just four minutes thanks to Riyad Mahrez's thunderous strike.
Hoffenheim, the current German league leaders, raced into a 2-0 lead with goals by defender Ermin Bicakcic and striker Munas Dabbur.
The veteran Brazil defender joined Frank Lampard's side from Paris Saint-Germain on a free transfer last month after suffering defeat to Bayern Munich...
The result is the highest win for an opening game of a new Bundesliga season.
Thiago agreed a "long-term contract" with Liverpool after deciding to end his seven-year stay with Bayern.
Ivan Perisic, Philippe Coutinho and Alvaro Odriozola have returned to Inter Milan, Barcelona and Real Madrid respectively after their loan deals expired....
Dortmund have spent the summer rebuffing interest from United and have insisted again and again they will not sell the England winger.
AFP Sport looks at five new signings to look out for this season.
Messi helped set up Philippe Coutinho, back from his loan at Bayern Munich, for the first goal after 21 minutes.
Sign up to receive our free newsletter